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. 2020 Jul 16;117(5):1358–1371. doi: 10.1093/cvr/cvaa207

Figure 2.

Figure 2

High salt induces a pro-inflammatory human monocyte phenotype. Human monocytes were magnetically isolated and cultured for 48 h in media containing normal salt (150 mM NaCl) or high salt (190 mM NaCl). (A) Representative flow plots showing differences in subtypes of monocytes cultured in normal or high-salt media (n = 13). (B) Paired comparison of percentages of live cells, (C) intermediate monocytes, and (D) non-classical monocytes. Panels E–G (n = 8) shows the effect of increasing sodium concentrations on conversion to intermediate (CD14++CD16+) monocytes, CD86 expression and IsoLG-adduct formation. Monocytes were cultured in increasing sodium concentrations by adding 2, 4, 8, 16, 32, and 40 mM of NaCl to RPMI media. Paired T-test was employed in B–D. Repeated measures one-way ANOVA with Dunnets post hoc test were employed for E–G. Clinical data of cell donors are shown in Supplementary material online, Table S5.