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. 2020 Jul 16;117(5):1358–1371. doi: 10.1093/cvr/cvaa207

Figure 5.

Figure 5

High salt drives monocytes to differentiate into a dendritic cell-like phenotype. Monocytes were isolated from the buffy coats of human volunteers and exposed to normal salt (NS), mannitol (Mann), or high salt (HS). (A) Immunofluorescence microscopic image showing morphology and CD209 staining of cells exposed to these conditions and also to a combination of GM-CSF and IL-4 after 6 days in culture (scale bars = 20 µm). (B) Flow cytometry gating strategy to identify monocytes. Flow cytometry representatives and average data (n = 10) showing the effect of high-salt exposure on dendritic cell markers CD1c (C) and CD83 (D). (E) Production of GM-CSF and Flt3 in media using a Luminex based assay (n = 8 and 12). Effect of high salt on IL-4 (F), GM-CSF (G), and FLT3 receptor (H) mRNA expression in monocytes using PrimeFlow (n = 4) (*P < 0.05). Data were analysed using paired t-tests. Clinical data of cell donors are shown in Supplementary material online, Table S5.