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. 2021 Mar 25;10(4):513. doi: 10.3390/antiox10040513

Figure 2.

Figure 2

Effects of TCS on reactive oxygen species (ROS) generation (B) in RBL-2H3 cells after compound 48/80 stimulation and nitric oxide (NO) production (D), inducible nitric oxide synthase (iNOS) and nuclear factor erythroid 2-related factor (Nrf2) expression levels (E) in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. (A) RBL-2H3 cells were seeded on 96-well plates and treated with TCS (1–100 μM) for 96 h to perform a water-soluble tetrazolium-1 (WST-1) assay. (B) RBL-2H3 cells pretreated with TCS for 24 h in compound 48/80 induced RBL-2H3 cells. The level of 2′,7′-dichlorodihydrofluorescein (DCF) fluorescence is presented as mean ± SD of three independent experiments. * p < 0.05 vs. control; # p < 0.05 vs. compound 48/80-treated cells. (C) RAW264.7 cells were treated with TCS for 24 h for cell viability assay. (D) RAW264.7 cells were pretreated with TCS for 3 h, followed by incubation with or without LPS (100 μg/mL) for 24 h. Data are expressed as mean ± SD of three independent experiments. * p < 0.05 vs. control; # p < 0.05 vs. LPS-treated cells. (E) RAW264.7 cells were pretreated with varying amounts of TCS (10, 25, 50, and 100 μM) for 4 h, then treated with LPS (100 μg/mL) for 24 h and subjected to Western blotting analysis with iNOS, Nrf2, and β-actin.