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. 2021 Mar 25;10(4):513. doi: 10.3390/antiox10040513

Figure 4.

Figure 4

TCS induces apoptosis of CT26 cells. (A) Cells were incubated with TCS for 48 h and stained with Annexin V and 7-AAD. The image is representative of three independent experiments. (B) Percentage of apoptotic cells. Results are expressed as the mean ± SD of three independent experiments. * p < 0.05. (C) CT26 cells were treated with various concentrations of TCS (10, 25, 50, and 100 μM) for 48 h and subjected to Western blotting analysis with antibodies against caspase-3, cleaved caspase-3, caspase-9, cleaved caspase-9, poly-ADP ribose polymerase (PARP), cleaved PARP, Bcl-2, Bcl-xL, Bcl-2-associated X protein (Bax), and β-actin. (D) The relative expression of apoptosis-related protein levels in TCS-treated CT26 cells were analyzed using the Image J program. Data are expressed as the mean ± SD of three independent experiments. * p < 0.05.