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. 2021 Mar 25;10(4):513. doi: 10.3390/antiox10040513

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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TCS regulates mRNA and protein expression of epithelial–mesenchymal transition (EMT) factors in CT26 cells. (A) Real-time qRT-PCR was performed to analyze the mRNA expression levels of EMT markers after treatment of TCS (0.25, 0.5, and 1 μM) on CT26 cells for 48 h. Data are the mean ± SD of three independent experiments. * p < 0.05. (B) The protein levels of EMT markers including E-cadherin, N-cadherin, vimentin, snail, and twist were confirmed by a Western blotting analysis. (C) Quantification was conducted via Image J. Data are the mean ± SD of three independent experiments. * p < 0.05.