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. 2021 Mar 25;10(4):513. doi: 10.3390/antiox10040513

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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TCS reduces the migratory and invasive abilities of CT26 cells. (A) Wound healing assay. Images were photographed using a microscope (200× magnification). Quantitative analysis for migration was measured by Image J as average fold change in width of the wound at 48 h compared to that at 0 h. (B) Invasion assay. Images of the invasion assay were photographed using a microscope (400× magnification). Photographs are representative of three independent experiments. Quantitative analysis for the invasion assay counted cells that invaded the membrane by Image J and presented as the relative ratio. (C) Gelatin zymography. Matrix metalloproteinase (MMP)-2 and MMP-9 activity in TCS-treated CT26 cells were determined. (D) mRNA expression levels of MMP-2 and MMP-9 were determined after TCS treatment for 48 h. Results are expressed as the mean ± SD of three independent experiments. * p < 0.05.