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. 2021 Mar 25;12(4):282. doi: 10.3390/insects12040282

Table 2.

Primer pairs used for qPCR analyses.

Primer Sequence 5’–3’ Annealing Temperature, °C Reference
Abaecin-F CAGCATTCGCATACGTACCA 60 [78]
Abaecin-R GACCAGGAAACGTTGGAAAC
Beta actin-F TTGTATGCCAACACTGTCCTTT 60 [79]
Beta actin-R TGGCGCGATGATCTTAATTT
ApidNT-F TTTTGCCTTAGCAATTCTTGTTG 60 [79]
ApidNT-R GTAGGTCGAGTAGGCGGATCT
Defensin-F TGCGCTGCTAACTGTCTCAG 60 [78]
Defensin-R AATGGCACTTAACCGAAACG
Hymenopt-F CTCTTCTGTGCCGTTGCATA 60 [78]
Hymenopt-R GCGTCTCCTGTCATTCCATT
VgMC-F AGTTCCGACCGACGACGA 60 [79]
VgMC-R TTCCCTCCCACGGAGTCC