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O-glycosylation of SARS-CoV-2 spike protein expressed in insect or human cells. (a) Experimental strategy. (b) A graphical layout of spike protein sequence annotated with identified O-glycosylation sites using Orbitrap Lumos mass spectrometer with ETD and HCD MS2 fragmentation. Independently identified O-glycosites on HEK 293F-derived ectodomain using Q Exactive HF-X mass spectrometer with HCD MS2 fragmentation are shown with orange outlines.
