Figure 4.

Effect of the FcHEx on the protein carbonylation in the skin keratinocytes. The keratinocytes were treated with the FcHEX or the positive control in the presence of 50 µM epinephrine for 24 h, fixed with formaldehyde, stained with DNPH (2,4-dinitrophenylhydrazine), and incubated with the specific antibody. The reported values represent the averages of three independent experiments and are expressed as the percentages of cells treated with epinephrine only, which was set to 100%. The asterisks indicate statistically significant values (*** p-value was between 0.0001 and 0.001; ** p-value was between 0.001 and 0.01).