Figure 6.

Effect of the FcHEx on the lipofuscin accumulation in the skin keratinocytes. The keratinocytes were treated with the FcHEx or the positive control for 24 h, then 50 nM of epinephrine was added to the cells incubated for an additional 48 h. At the end of the incubation, the cells were stained and the absorbance at 595 nM was measured. The obtained values were normalized relative to the protein content determined using a Bradford assay. The reported values represent the averages of three independent experiments and are expressed as the percentages of cells treated with epinephrine only, which was set to 100%. The asterisks indicate statistically significant values (*** p-value was between 0.0001 and 0.001; ** p-value was between 0.001 and 0.01).