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(A) IFN-γ–treated SKMEL28 cells analyzed for MANF secretion using ELISA and immunoblot analysis (B). (C-D) MANF expression at the mRNA level. PD-L1 served as positive control. (E) MANF knockdown in SKMEL28 cells assessed by immunoblot (left) and RT-PCR (right). Data are representative of at least three independent experiments. Expression in (C) and (D) is relative to baseline. N = 3, data is represented as mean ± SEM. ** p-value<0.01, *** p-value<0.001.
