Fig 2. IFN-γ induces MANF secretion in additional cell-lines.

MC38 (A and B) and HepG2 (C and D) cell-lines were treated with 100 ng/ml IFN-γ for 48 hours, followed by immunoblot analysis of the cells lysates and media (upper panels) or RT-PCR analysis of MANF and PD-L1 (lower panels). RNA expression is relative to baseline. N = 3, data is represented as mean ± SEM. *** p-value<0.001.