Fig 3. IL1B did not induce MANF secretion in SKMEL28.

SKMEL28 cells were treated with 50 ng/ml (+) or 100 ng/ml (++) IFN-γ and 5 ng/ml (+) or 20 ng/ml (++) IL1B for 48 hours, followed by immunoblot analysis of the cells and media (a), RT-PCR analysis of MANF and PD-L1 (b), and IL-8 ELISA analysis of the media (c). RNA expression is relative to baseline. N = 3, data is represented as mean ± SEM. *** p-value<0.001.