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. 2021 Apr 23;16(4):e0249838. doi: 10.1371/journal.pone.0249838

Fig 1. Administration of recombinant AIM improves graft function and mitigates graft damage.

Fig 1

Donor kidneys were transplanted into B6 wild-type recipients. Following transplantation, recipients were injected intravenously (i.v.) with either 200 μl of rAIM (2mg/ml) or PBS. On day 2 post-transplantation, mice were euthanized and (A) Serum creatinine was measured as a marker of renal function (B-C) Formalin-fixed tissue sections were stained with H&E and were scored in a blinded fashion. Scoring system: 0 = none, 1 = <10%, 2 = 11–25%, 3 = 26–45%, 4 = 46–75%, and 5 = >75%. (B) ATN score, (C) tubular obstruction score (D) apoptotic tubular epithelial cells were quantified. Tubular epithelial cell staining positive staining for cleaved Caspase-3-postive were counted and averaged from 5 different fields at 400x magnification for each section (E) Images of H&E -stained tissue sections. *p<0.05, **<p<0.01, n = 4~5/group (F). Formalin-fixed tissue sections were stained for necrosis using Periodic acid-Schiff (PAS) (G).