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. 2021 Apr 23;16(4):e0249838. doi: 10.1371/journal.pone.0249838

Fig 3. Recombinant AIM enhances the phagocytic uptake of necrotic cells by both KIM-1 expressing mouse and human kidney cells.

Fig 3

Renca cells (mouse) or HEK293 (human) expressing KIM-1 or without KIM-1 were fed necrotic thymocytes with or without rAIM (A-C). (A) Renca cells (mouse) after a 90-minute incubation, % phagocytosis was quantified using flow cytometry. (B) Immunotyping of necrotic cell populations. (C) Western Blots were performed confirming the expression of KIM-1 on Renca cells. (D) HEK 293 cells (human) after a 90-minute incubation, % phagocytosis was quantified using flow cytometry. (E) Western Blots were performed confirming the expression of KIM-1 on HEK293 cells. Histograms are representative results of at least 3 independent experiments. All flow cytometry experiments were analyzed using FlowJo X software. **p<0.01, ***p<0.001.