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. 2021 Apr 23;16(4):e0248380. doi: 10.1371/journal.pone.0248380

Fig 2. Characterization of TSC1 and TSC2 deficient cell lines.

Fig 2

Immunoblots of TSC1 and TSC2 deficient cell lines were performed to examine multiple components of the mTORC1 signaling pathway. Cells were serum starved for 24h (−) or had serum add back for 30 min (+) after starvation. CAL-72 and PEER cells showed no TSC1 and reduced TSC2 expression. SNU-878, SNU-886, and SNU-398 showed an absence of TSC2 expression. pS6K (Thr389), pS6 (Ser235/236 and Ser240/244) p4E-BP1 (Thr37/46), and pEIF2 (Ser51) showed a strong expression in a serum starved condition in all TSC1 or TSC2 deficient cell lines, indicating constitutive mTORC1 activation. GAPDH and BIP were used as loading controls.