Skip to main content
. 2021 Apr 23;16(4):e0248380. doi: 10.1371/journal.pone.0248380

Fig 4. Expression and signaling effects of TSC2 add back to TSC2 deficient cells.

Fig 4

TSC2 cDNA or an empty vector was added to TSC2 deficient cell lines via retroviral transfection. Cells were serum starved for 24h (−) or had serum add back for 30 min (+). In the TSC2 add back cells there is a strong TSC2 expression, a decreased pS6 (S240/244) expression in the absence of serum, and increased pAKT (Ser473) expression following serum add back. No pAKT (Ser473) was seen in SNU-398 cells, with or without TSC2 addback.