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. 2021 Apr 23;16(4):e0248380. doi: 10.1371/journal.pone.0248380

Fig 5. Impact of rapamycin and Torin1 on constitutively activated mTOR signaling pathway in TSC1 or TSC2 deficient cells.

Fig 5

Cells were treated with Torin1 (250 nM) or rapamycin (20 nM) for 24h. Untreated cells and MGH-U1 were used as controls. Cells were serum starved for 24h (−) or received after starvation serum add back for 30 min (+). Rapamycin and Torin1-treated cells show no expression of pS6K (Thr389) and pS6 (Ser 240/244) in serum absence or stimulated conditions compared to the upregulated expression in untreated cells. pAKT (Ser 473) levels are lower after Torin1 and higher after rapamycin treatment.