Fig 6. Effect of ganetespib on protein expression and mTORC1 signaling in TSC-mutant cell lines.

Cells were treated with ganetespib in increasing concentrations (1, 10, 100 nM, and 1 μM) for 24h. Cells were serum starved for 24h (−) or after starvation had serum add back for 30 min (+). Effects on mTOR, AKT, and pS6 (Ser240/244) expression were seen at 0.1 and 1mM.