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. 2021 Apr 23;16(4):e0248380. doi: 10.1371/journal.pone.0248380

Fig 7. Impact of rapamycin, Torin1, and ganetespib on constitutively activated mTOR signaling and cleaved caspase 3.

Fig 7

Cells were treated with rapamycin (20 nM), Torin1 (250 nM) or ganetespib (100 nM) for 24h. Cells were serum starved for 24h (−) or received after starvation serum add back for 30 min (+). Cells treated with rapamycin do not show expression of pS6 (Ser240/244) and expression of p4E-BP1 isoforms are reduced due to mTORC1 inhibition. Torin1-treated cells show no pS6 (Ser240/244), p4E-BP1, and a lowered pAKT (Ser473) expression, due to mTORC1/2 inhibition. Ganetespib-treated cells show a lowered AKT, S6K, and pS6 (Ser240/244) expression. The expression of cleaved caspase 3 is very distinctive among different cell lines.