Table 1. CCLE cell lines with reported nonsense mutations, frameshift deletions or insertions, or in-frame deletions in TSC1 and TSC2 [adapted from 27, 28].

tumor sample	allele ratio var/ total	genome change, hg19	variant classification	variant type	reference allele	tumor seq. allele	cDNA change	codon change	protein change
TSC1, chromosome 9
PEER_HAEM_AND_LYMPH	1.00	g.chr9:135798751C>T	Nonsense_Mutation	SNP	C	T	c.492G>A	c.(490–492)TGG>TGA	p.(Trp164*)
KM12_LARGE_INTESTINE	0.56	g.chr9:135797261_135797261delA	Frame_Shift_Del	DEL	A	-	c.608_608delT	c.(607–609)TTGfs	p.(Leu203Cysfs*7)
2313287_STOMACH	0.52	g.chr9:135785954G>A	Intron	SNP	G	A	c.1263+4C>T
CW2_LARGE_INTESTINE	0.47	g.chr9:135781157_135781157delG	Frame_Shift_Del	DEL	G	-	c.1808_1808delC	c.(1807–1809)CCGfs	p.(Pro603Argfs*26)
TSC2, chromosome 16
NCIH1651_LUNG	0.33	g.chr16:2100401G>T	Nonsense_Mutation	SNP	G	T	c.139G>T	c.(139–141)GAA>TAA	p.(Glu47*)
CW2_LARGE_INTESTINE	0.60	g.chr16:2108787_2108788insT	Frame_Shift_Ins	INS	-	T	c.888_889insT	c.(886–891)GTGTTTfs	p.(Val299Cysfs*39)
HEC151_ENDOMETRIUM	0.42	g.chr16:2121790_2121791insAG	Frame_Shift_Ins	INS	-	AG	c.1952_1953insAG	c.(1951–1953)CCAfs	p.(Gly654Glufs*45)
DV90_LUNG	0.36	g.chr16:2121791_2121792delAG	Frame_Shift_Del	DEL	AG	-	c.1953_1954delAG	c.(1951–1956)CCAGAGfs	p.(Gly654Leufs*2)
OVK18_OVARY	0.67	g.chr16:2121791_2121792delAG	Frame_Shift_Del	DEL	AG	-	c.1953_1954delAG	c.(1951–1956)CCAGAGfs	.(Gly654Leufs*2)
MFE319_ENDOMETRIUM	0.42	g.chr16:2122880C>T	Nonsense_Mutation	SNP	C	T	c.2251C>T	c.(2251–2253)CGA>TGA	p.(Arg751*)
SNU-886_LIVER	0.97	g.chr16:2129160C>T	Nonsense_Mutation	SNP	C	T	c.3094C>T	c.(3094–3096)CGA>TGA	p.(Arg1032*)
SNU-878_LIVER	1.00	g.chr16:2134999C>G	Nonsense_Mutation	SNP	C	G	c.4541C>G	c.(4540–4542)TCA>TGA	p.(Ser1514*)
HEC151_ENDOMETRIUM	0.45	g.chr16:2138565G>A	Missense_Mutation	SNP	G	A	c.5378G>A	c.(5377–5379)CGG>CAG	p.(Arg1793Gln)
HCC95_LUNG	0.60	g.chr16:2138578_2138580delCTC	In_Frame_Del	DEL	CTC	-	c.5391_5393delCTC	c.(5389–5394)ATCTCC>ATC	p.(Ser1799del)