Fig 3. Production of prostaglandin E₂ (PGE₂) and its metabolites by fetal-membrane in vitro.

(A) Schematic presentation of fetal-membrane Transwell assay. (B-I) Accumulation of PGE₂ (B and C),15-keto-PGE₂ (D and E), 13,14-dihydro-15-keto-PGE₂ (F and G) and 19-hydroxy-PGE₂ (H and I) in maternal and fetal compartments was quantified by LC-MS/MS. Circle markers and error bars indicate the mean ± SEM. Data were analyzed by two-way ANOVA. *p <0.05, **p <0.01, ***p <0.005, ****p <0.0001. Term not in labor (TNL) samples, n = 6; term in labor (TLB) samples, n = 6.