Figure 4.

TLR4 deletion reverses HMGB1-induced polarization towards the Th1 phenotype. (A) The CCK-8 method was used to detect the effects of CD4⁺CD25⁺ Tregs on the proliferation of CD4⁺CD25⁻ T cells from different groups. (B) Changes in IL-2 contents in cocultures of CD4⁺CD25⁺ Tregs and CD4⁺CD25⁻ T cells. (C) IL-4 contents in cocultures of CD4⁺CD25⁺ Tregs and CD4⁺CD25⁻ T cells. (D) IFN-γ contents in cocultures of CD4⁺CD25⁺ Tregs and CD4⁺CD25⁻ T cells, *p<0.05 between 3 groups by using Tukey’s test, and ^&p<0.05 between WT and TLR4^-/- group by using t-test.