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. 2021 Apr 19;14:2711–2726. doi: 10.2147/OTT.S280490

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© 2021 Han et al.

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CNTN1 was a target gene of miR-1305. (A) Fragments of CNTN1-WT and CNTN1-Mut containing the binding site for miR-1305. (B) Dual-luciferase reporter assay. **p<0.01. (C) Western blot for CNTN1 protein expression in A2780 and SKOV3 cells after transfection. **p<0.01. (D) Western blot for CNTN1 protein expression in A2780-DDP and SKOV3-DDP cells after transfection. **p<0.01. (E) qRT-PCR for CNTN1 mRNA expression in clinical tissues. (F) Pearson’s correlation analysis for LINC00184 and CNTN1 mRNA expression in clinical tumor specimens. (G) Pearson’s correlation analysis for miR-1305 and CNTN1 mRNA expression in clinical tumor specimens. (H) Western blot was used to research CNTN1 protein expression in A2780, SKOV3, A2780-DDP and SKOV3-DDP cells. **p<0.01 relative to A2780-DDP cells. ##p<0.01 relative to SKOV3-DDP cells. All experiments were performed in triplicate.