Key resources table.

Reagent type (species) or resource	Designation	Source or reference	Identifiers	Additional information
Genetic reagent (Mus musculus)	ChAT-Cre	The Jackson Laboratory	Cat. #: 006410; RRID:MGI:3689420	Dr Bradford Lowell
Genetic reagent (Mus musculus)	Ai32	The Jackson Laboratory	Cat. #: 012569; RRID:MGI:104735	Hongkui Zeng, Allen Institute for Brain Science
Antibody	Anti-GFP (chicken polyclonal)	Abcam	Cat. #: AB13970; RRID:AB_300798	IHC (1:1000)
Antibody	Anti-ChAT (goat polyclonal)	Millipore	Cat. #: AB144; RRID:AB_90650	IHC (1:500)
Antibody	Anti-chicken Alexafluor488	Life Technologies	Cat. #: A11039; RRID:AB_142924	IHC (1:400)
Antibody	Anti-goat Alexafluor594	Abcam	Cat. #: AB150132; RRID:AB_2810222	IHC (1:1000)
Software, algorithm	R	R Project for Statistical Computing	RRID:SCR_001905
Software, algorithm	MATLAB	MATLAB	RRID:SCR_001622
Software, algorithm	IGOR Pro	IGOR Pro	RRID:SCR_000325