Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Homo sapiens) | PLIN1 | Jacquier et al., 2013 | PLIN1_HUMAN AAH31084.1 |
Supplementary file 1 |
| Gene (Homo sapiens) | PLIN2 | Jacquier et al., 2013 | PLIN2_HUMAN AAH05127.1 |
Supplementary file 1 |
| Gene (Homo sapiens) | PLIN3 | Jacquier et al., 2013 | PLIN3_HUMAN AAC39751.1 |
Supplementary file 1 |
| Strain, strain background (Escherichia coli) | BL21(DE3) | ThermoFisher | C600003 | Chemically competent cells |
| Strain, strain background (Saccharomyces cerevisiae) |
BY4742 | Euroscarf | MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0 | |
| Strain, strain background (S. cerevisiae) | pet10Δ | Euroscarf | MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0 pet10Δ::KANMX4 | |
| Strain, strain background (S. cerevisiae) | Pet10-GFP | Huh et al., 2003 | MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0 PET10-GFP::HisMX | |
| Cell line (D. melanogaster) | S2 | ThermoFisher | R69007 | |
| Cell line (Homo sapiens) | HeLa | ATCC | CCL-2 | |
| Antibody | Anti-GFP (rabbit polyclonal) | Thermo Fisher Scientific | A11122 | (1:5000) |
| Antibody | Anti-rabbit (goat polyclonal, HRP conjugate) | Sigma-Aldrich | A6154 | (1:5000) |
| Antibody | Anti-Vps10 (mouse monoclonal) | Molecular probes | A-21274 | (1:100) |
| Antibody | Anti-mouse (donkey, HRP conjugate) | GE Healthcare | NA934V | (1:10000) |
| Peptide, recombinant protein | Plin4 12mer | Čopič et al., 2018 | Human Plin4 (aa510-905) | Supplementary file 2 |
| Peptide, recombinant protein | Plin4 4mer | Čopič et al., 2018 | Human Plin4 (aa246-377) | Supplementary file 2 |
| Peptide, recombinant protein | Plin4(4T > S) (4mer) |
This study | Supplementary file 2 | |
| Peptide, recombinant protein | Plin4(NN) (4mer) |
This study | Supplementary file 2 | |
| Peptide, recombinant protein | Plin4 csw (12mer) |
This study | Supplementary file 2 | |
| Peptide, recombinant protein | Plin3 AH | This study | Human Plin3 (aa113 – 205) | Supplementary file 2 |
| Chemical compound, drug | SyproOrange | ThermoFisher | S6651 | |
| Chemical compound, drug | Alexa488 C5 maleimide | ThermoFisher | A10254 | |
| Chemical compound, drug | Alexa568 C5 maleimide | ThermoFisher | A20341 | |
| Chemical compound, drug | Bodipy 493/503 | ThermoFisher | 11540326 | |
| Chemical compound, drug | Diphytanoyl-phosphatidylserine | Avanti Lipids | AVA-850408C-25Mg | |
| Chemical compound, drug | Diphytanoyl-phosphatidylcholine | Avanti Lipids | AVA-850356C-200Mg | |
| Other | Glass microfluidic chip with a T-junction | Dolomite | part # 3000086 | |
| Other | Glass microfluidic chip with a T-junction | Dolomite | part # 3000024 | |
| Other | Silica Microspheres,5.00 μm, SS05N | Bang laboratories | SS05003-0.5 |