Table 3.
The list of Rattus norvegicus primers used for quantification of mRNA expression levels in qRT-PCR.
| Sr. No. | Name of the gene | Forward primer sequence | Reverse primer sequence |
|---|---|---|---|
| 1. | 18S | GTTGGTTTTCGGAACTGAGGC | GTCGGCATCGTTTATGGTCG |
| 2. | CYP2E1 | TCAATCTCT GGACCCCAACTG | GCGCTCTGCACTGTGCTTT |
| 3. | TLR-4 | TTGAAGACAAGGCATGGCATGC | TCTCCCAAGATCAACCGATG |
| 4. | TNF-α | TCTCATTCCTGCTCGTGGCG | GGTGAGGAGCACGTAGTCGG |
| 5. | IL-6 | TTGACAGCCACTGCCTTCCC | CGGAACTCCAGAAGACCAGAGC |
| 6. | HO-1 | CAAATCCCACCTTGAACACA | CGACTGACTAATGGCAGCAG |
| 7. | Nrf-2 | CAGAGTTTCTTCGCCAGAGG | TGAGTGTGAGGACCCATCG |
| 8. | PPAR-γ | ACGATCTGCCTGAGGTCTGT | CATCGAGGACATCCAAGACA |
| 9. | HSL | AGTGAAAAACCCGCGGACC | TTCATCCTTCTGCCCCCTAC |
| 10. | AMPK | GCTGTGGATCGCCAAATTAT | GCATCAGCAGAGTGGCAATA |
| 11. | SREBP-1c | TCTGCCTTGATGAAGTGTGG | AGCAGCCCCTAGAACAAACA |
| 12. | ACC | CCTTCTTCTACTGGCGACTGAG | TAAGCCTTCACTGTGCCTTCC |
| 13. | FAS | CCTCAGTCCTGTTATCACCCGA | GCTGAATACGACCACGCACTA |
| 14. | IL-10 | TGCCTTCAGTCAAGTGAAGAC | AAACTCATTCATGGCCTTGTA |