Figure 7.
Crystal structure and key interactions of 47D1 in complex with SARS-CoV-2 RBD
(A) 47D1 (cyan and pale cyan for heavy and light chains, respectively) in complex with the RBD (white).
(B) 47D1 binding to RBD relative to RBD-ACE2 binding.
(C and D) Epitope of 47D1. Epitope residues contacting the heavy chain are shown in dark green and those contacting the light chain are shown in light green. CDR loops and the framework region that contact the RBD are labeled. (D) Epitope residues that are important for binding to 47D1 are labeled. Epitope residues are defined here as residues in SARS-CoV-2 RBD with buried surface area (BSA) > 0 Å2 after Fab 47D1 binding, as calculated with Proteins, Interfaces, Structures and Assemblies (PISA) (Krissinel and Henrick, 2007). For clarity, only representative epitope residues are labeled.
(E–G) Interactions between SARS-CoV-2 RBD and (E) CDR H1, (F) CDR H2, and (G) CDR H3 and L1. The heavy and light chains of 47D1 are shown in cyan and pale cyan, respectively. The RBD is in white. Hydrogen bonds are represented by dashed lines.
See also Figures S3–S6 and Tables S4 and S5.