REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
APC-Cy7 Anti-Human CD3 (Clone SP34-2)	BD Biosciences	Cat#557757; RRID: AB_396863
APC/Cy7 Anti-human CD4 antibody (Clone OKT4)	BioLegend	Cat#317418; RRID: AB_571947
APC-Cy7 Anti-Human CD8 (Clone RPA-T8)	BD Biosciences	Cat#557760; RRID: AB_396865)
APC/Cy7 Anti-human CD14 (Clone M5E2)	BD Biosciences	Cat#561384; RRID: AB_10611720
PerCP/Cy5.5 Anti-human CD19 (Clone HIB19)	BD Biosciences	Cat#561295; RRID: AB_10644017
PerCP/Cy5.5 Anti-human CD20 (Clone 2H7)	BioLegend	Cat#302326; RRID: AB_893283
PE anti-human IgM (Clone MHM-88)	BioLegend	Cat#305212; RRID: AB_314508
PE anti-human IgD (Clone IA6-2)	BioLegend	Cat#348204; RRID: AB_10553900
BV421 anti-human IgG (Clone G18-145)	BD Biosciences	Cat#562581; RRID: AB_2737665
F(ab’)2 goat anti-human IgG+IgM (H+L)	Jackson ImmunoResearch	Cat#109-006-127; RRID: AB_2337552
SARS-CoV-2 Spike Glycoprotein (944-1218aa) antibody	Proteintech	Cat#28867-1-AP; RRID: AB_2881223
Unconjugated goat anti-human IgG (H+L) antibody	Jackson ImmunoResearch	Cat#109-006-088; RRID: AB_2337549
Alkaline Phosphatase AffiniPure F(ab’)2 Fragment Goat Anti-Human IgG	Jackson ImmunoResearch	Cat#109-056-098; RRID: AB_2337618
Bacterial and virus strains
SARS-CoV-2 strain USA-WA1/2020	BEI Resources	Cat#NR-52281
SARS-CoV-2 pseudotyped virus	This paper	N/A
Turbo Competent E. coli	NEB	Cat#C2984H
Biological samples
PBMCs from COVID-19 convalescent patients	Affiliated Hospital of Putian University	N/A
Chemicals, peptides, and recombinant proteins
SuperScript IV Reverse Transcriptase	Thermo Fisher	Cat#18090050
Streptavidin-AF647	Thermo Fisher	Cat# S12374
Streptavidin-AF488	Thermo Fisher	Cat# S11223
TurboCapture 96 mRNA Kit	QIAGEN	Cat# 72251
HotStarTaq DNA Polymerase	QIAGEN	Cat# 203203
Critical commercial assays
Bright-Glo Luciferase Assay System	Promega	Cat# E2620
Deposited data
X-ray coordinates and structure factors for 47D1/RBD	This study	PDB: 7MF1
Experimental models: cell lines
HeLa cells	ATCC	Cat# CCL-2
293T cells	ATCC	Cat# ACS-450
HeLa-ACE2 cells	This study	N/A
Expi293F cells	ThermoFisher	Cat# A14527
Vero cells	ATCC	Cat# CCL-81
Experimental models: organisms/strains
Golden Syrian Hamster	Charles river	N/A
Recombinant DNA
pcDNA-SARS-CoV-2-S-Δ18aa	(Rogers et al., 2020)	N/A
Software and algorithms
FlowJo	BD Biosciences	N/A
GraphPad Prism 8	GraphPad	N/A
PyMOL	Schrödinger, LLC	RRID: SCR_000305
Octet analysis software 9.0	Fortebio	https://www.moleculardevices.com
IMGT	International ImMunoGeneTics Information System	http://www.imgt.org
Phaser	(McCoy et al., 2007)	N/A
Other
Ni-NTA biosensors	ForteBio	Cat# 18-5102
BD FACSAria Fusion Cell Sorter	BD Bioscience	N/A
STROM Super-Resolution Microscope System	Nikon	N/A