Table 1.

Dilution series of SARS-CoV-2 and LamPORE

Concentration (copies/mL)	ORF1ab reads (median)	E1 reads (median)	N2 reads (median)	RT-qPCR result	ORF1ab Ct	N Ct
20 000	6429	808	2288	Detected	10.9	14.7
2000	1385	18	602	Detected	28.1	24.1
200	27	4	82	Detected	30.7	30.7
20	67	6	979	Detected	N/D	33
2	0	0	45	Not detected	N/D	N/D
0.2	16	0	0	Not detected	N/D	N/B

Abbreviations: Ct, cycle threshold; E1, envelope protein 1 gene; LamPORE, loop-mediated isothermal amplification (LAMP) coupled to nanopore sequencing technology; N2, nucleocapsid protein 2 gene; RT-qPCR, quantitative RT-PCR; SARS-CoV-2, severe acute respiratory symdrome coronavirus 2.