FIG. 6.

Loss of labeling by CB2 primary antibody omission and immunizing peptide block. Representative sample images are shown at 5×(A–D) and 40×(E–H) magnification in the superficial dorsal horn of sections with the primary antibody omitted (A, E) or sections incubated overnight with the optimal concentration of CB2 primary (1:250; B–D and F–H). Some sections (C, D, and G, H) were incubated with antibody that had been preincubated (30 min) with immunizing peptide corresponding to the amino acid binding sequence targeted by the antibody.