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. 2021 Jan 9;108(5):667–679. doi: 10.1007/s00223-020-00793-1

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© The Author(s) 2021

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Fig. 1 — Human amniotic membrane. Histological cross-sections of the fresh and intact amniotic membrane before decellularization presented the epithelial cell layer (arrow, a) with purple nuclei, removed during the process which preserved the collagenous matrix (b). H&E stain, Objective × 20, Scale bars 100 µm. Decellularized membrane inside a T75 Cell Culture Flask (c)