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. 2021 Jan 9;108(5):667–679. doi: 10.1007/s00223-020-00793-1

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 9 — Details of DAM scaffolds in calvarial bone repair. Bone (arrowhead) deposited in the concave area between two transplanted DAM layers (arrow) (a). Osteoconduction established by vascularization between amniotic membrane layers (arrows), cell migration between collagenous fibers of the amniotic membrane, and creation of niches for bone deposition (arrowhead) (b). PRS-POL image of the specimen from b exhibits the DAM as bright red fiber agglomerate (arrow), interposed by bright yellow endogenous collagen (arrowhead) (c). PRS-POL images of specimens from three groups: T0 without treatment (d), T1 treatment with DAM scaffold (e), and T2 with DAM associated with transplanted ASCs (f), with the meningeal side toward the bottom of the section. Endogenous fibrous tissue from T0 (d), displaying upper and lower parts collapsed (asterisk), without matrix in the middle. Treatments with DAM (e T1; f T2) exhibit the transplanted collagenous DAM as bright red fiber agglomerate (arrow) interposed between upper and on lower fibrous tissue (asterisk), and bright green/yellow endogenous collagen and bone (arrowhead) deposited in the middle. H&E stain (a, b), PRS stain (c–f); Objective × 20 (a, b), Objective 10X (c–f); Scale Bars 100 µm