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Sequences of primers designed for Identification of SCs by reverse transcription-polymerase chain reaction (RT-PCR)
| Gene name | bp | Primers sequences | Melt (°C) |
|---|---|---|---|
| Plzf | 137 | F: CCCGTTGGGGGTCAGCTAGA | 61 |
| R: CTGCAAGGTGGGGCGGTGTAG | |||
| Gfrα1 | 130 | F: CTGTGGACTAGCTCGCTCTC | 60 |
| R: GACCCGCTTTTAGGGGTTCA | |||
| Id4 | 185 | F: GGGTGACAGCATTCTCTGC | 58.52 |
| R: TTGGAATGACAAGACGAGACG | |||
| Gapdh | 125 | F: CTGCTGGACAAGTGAGTCCC | 60 |
| R: CCAAGTACCCTGGCCTCATC |
