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. 2021 Apr 23;8:115. doi: 10.1038/s41597-021-00890-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — Validation of the normalization procedure by comparison to absolute protein abundances in six cell lines. Panel (a) Examplary relationship between estimated absolute protein abundances in copies per cell (cpc) and ibaq or ibaq.ppm.svd.imputed.batch quantification values in the MCF7 cell line. Panel (b) Distributions of the calculated Spearman’s rank correlation coefficients between absolute protein abundances and quantification values based on various normalization and missing data imputation methods.