Fig. 2. CA521^FALA shows modified affinity to various Fc or complement receptors.

a–f Affinity of CA521(IgG1) and CA521^FALA to FcγRI, FcγRIIA R167, and FcγRIIB/C. It was assessed by the surface plasmon resonance (SPR) assay using the BIAcore 8k system. Experiments were performed in duplicate and one representative data were displayed. g–i ADCP analysis of CA521 ^FALA. Target cells were stained with CFSE and macrophages were stained with APC-anti-CD206. Double stained macrophages are considered to have phagocytized target cells. Phagocytosis rate (in red font) = Q2-2%/(Q2-1% + Q2-2%)*100% (double-stained macrophages/APC-anti-CD206 stained macrophages). Phagocytosis rates for CA521(IgG1), isotype, and CA521^FALA were 12.74, 3.76, and 3.33%, respectively. Experiments were performed two times and one representative data was displayed.