Fig. 8.

AICAR could up-regulate the protein expression of key genes in the AMPK-PGC-1α signaling pathway after HS 2 d. The cells were treated with HS (41.5 °C) or AICAR (0.2 mM) for 2 d. a-d The expression of AMPK, P-AMPK and PGC-1α protein abundance were detected by Western blot. Numbers represent mean ± SEM (n = 3). Means with different letters were significantly different at P < 0.01