Table 1.
Primers used in this study
| Application of primers | Gene | Forward primer (5′-3′) | Reverse primer (5′-3′) |
|---|---|---|---|
| Full-length confirmation | Hsp21.3 | GCGTAGCGACACTGTGATTC | CTCTGCTCAATGCAGGTTGTG |
| Hsp20.0 | CCCGCCGGCAAAACATTCA | TCACTTAGCTGTTCCGTTGGC | |
| Hsp20.1 | CATTCAACTGAACGCGACACT | AGCAAGCTCAGCTCGACAG | |
| Hsp19.3 | CGCGAATACAACAACACAACAA | CATACAAACTTAACACAATTAAGGA | |
| Hsp29 | GCAGCCGGCATAGTACATTA | ACGCTTTAATGACTGTCGGT | |
| qPCR analysis | Hsp21.3 | TCGACACTGAATTCTCCAGCA | TCTGCCAACTGTCTGCTGTC |
| Hsp20.0 | GACAGCTGATGGCTACGTGA | GCGATGACTGTCAAGACACC | |
| Hsp20.1 | CAGCCGCGACTACTACAGAC | TCCTCGTGCTTAGCTTCCAC | |
| Hsp19.3 | TGGACCAGAACTTCGGACTG | ACATCCACGTTGATCTGCCAT | |
| Hsp29 | TTCATCACCACCGTAGCCTG | TGATGAGTTGACTCCACGGC | |
| RPL27 | GAAGCCAGGTAAAGTGGTGCT | GTGTCCGTAGGGCTTGTCTG | |
| β-actin | GATCTGGCACCACACCTTCT | GGCGTGTTGAAGGTCTCGAA |