Fig. 8. IPLD treatment of orthotopic and lung metastatic dual tumours of 4T1 triple-negative breast cancer.

a Experimental schedule for the dual tumour model: BALB/c mice were s.c. inoculated with 1 × 10⁶ 4T1 cells at the left mammary gland; when the tumours reached about 100 mm³, the treatments were initiated (noted as Day 0) with the liposome formulas at 7.5 mg kg⁻¹ IOX1, 5 mg kg⁻¹ DOX or their combination every 2 days for three times; 3 days after the last treatment (Day 7), ^Luci 4T1 cells (5 × 10⁵) were injected to the mice via the tail vein. b The serum IFN-γ and TNF-α levels in the mice determined on day 9; n = 3 mice. c The tumour growth curves of the orthotopic 4T1 tumours; n = 5 mice. d The individual tumour growth of each mouse in the IPLD-treated group; CR: complete response. e The images of the tumours dissected at the end of the experiment (Day 22). f In vivo live bioluminescence imaging the ^Luci4T1 tumours in lungs on day 22; n = 5 mice. g Representative photographs of the lungs dissected from the mice on day 22. Data represent mean ± SD. Two-tailed Student’s t test. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. Source data are provided as a Source Data file.