Skip to main content
. 2021 Apr 23;11:8828. doi: 10.1038/s41598-021-87828-1

Figure 1.

Figure 1

Effects of LPS and JQ1 on HMC3 cells and the expression of inflammatory response-related genes in LPS- and JQ1-treated cells. (A) Morphology of HMC3 cells after treatment with LPS, JQ1, and LPS + JQ1 for 4 h. (B) HMC3 cells were cultured for 4 h after treatment with LPS (100 ng/ml) and JQ1 (500 nM) and stained for IBA1 (green fluorescence), a microglia-lineage marker. Nuclei were counterstained with DAPI (blue). (C) HMC3 cells were treated with LPS and JQ1 at different concentrations for different durations (4 h, 24 h, and 48 h). The viability of the HMC3 cells was determined using the WST-1 assay. (D) HMC3 cells were treated with LPS at different doses (10, 100, and 1000 ng/ml) for 4 h. Inflammatory genes were significantly upregulated in cells treated with LPS compared to DMSO-treated control cells (upper panel). HMC3 cells were treated with LPS (100 ng/ml) and JQ1 at different doses (50, 500, and 5000 nM) for 4 h. Inflammatory genes were significantly downregulated in cells treated with JQ1 (bottom panel). Gene expression was normalized to GAPDH transcript levels. The data represent three independent experiments. The values are the mean ± SEM of triplicate experiments (*p < 0.05 and **p < 0.001).