Figure 2.

Differentially expressed genes in LPS- and LPS + JQ1-treated HMC3 cells. (A) Heat map showing the top 50 upregulated genes in HMC3 cells treated with LPS and LPS + JQ1 for 4 h determined by RNA-seq (p ≤ 0.05 and fold change ≥ 1.5 log2). Each experiment was performed in experimental triplicates (n = 3) for each condition, and the results were individually combined. The color scale shown in the heat map represents the log₂ fold change values. Red cells indicate upregulated gene, while blue cells indicate downregulated genes. The p-value with an asterisk attached in the cell represents *p < 0.05, **p < 0.01, and ***p < 0.001. (B) GO term analysis of the biological processes associated with up- and downregulated genes after LPS (100 ng/ml) and JQ1 (500 nM) treatment for 4 h. GO analysis of the number of genes shown in the van diagram. (C) Biological pathway analysis of DEGs in cells treated with LPS and LPS + JQ1 using IPA (QIAGEN Inc., https://www.qiagenbioinformatics.com/products/ingenuitypathway-analysis) showed up- and downregulated genes.