Figure 3.

JQ1 inhibits a specific subset of LPS-inducible inflammatory genes. (A) Heat map showing chemokine/cytokine and interferon response gene expression in HMC3 cells treated with LPS (100 ng/ml) and JQ1 (500 nM) for 4 h. The color scale shown in the heat map represents the log₂ fold change values. (B) IPA downstream effects analysis presenting the mapping of most significant genes related to inflammatory response genes in LPS-treated cells. The DEGs are colored by predicted activation state, such as activated (red) or suppressed (green). The edges connecting the nodes are colored orange (leading to activation of the downstream node), blue (leading to inhibition), and yellow (if the findings underlying the relationship are inconsistent with the state of the downstream node). (C) Expression levels of inflammation-related genes were analyzed by qRT-PCR and normalized to GAPDH transcript levels. Overall, LPS-induced inflammatory genes were inhibited by JQ1. The data represent three independent experiments. The values are the mean ± SEM of triplicate experiments (**p < 0.001).