Fig. 1. CRISPR/Cas9 targeting of PDX1 protein-coding sequence in primary human islets.

A Schematics of the human pseudoislet system. B Scheme of the lentiCRISPR construct used. C PDX1 sequence, showing the sgRNA sequence (underlined in red, PAM sequence recognized by Cas9 in red box). FAM (blue) and HEX (orange) probes used for ddPCR. D Percentage of indel-modified sequences detected by the TIDE algorithm (P = 0.01). E–H Immunostaining of CRISPR-Control versus CRISPR-PDX1, showing GFP⁺ PDX1⁺ cells (n = 3 independent donor repetitions); scale bar: 50 μm. I Quantification of GFP⁺ PDX1⁺ cells for CRISPR-Control versus CRISPR-PDX1(P = 0.07; n = 3 independent donor repetitions). Data are presented as mean values ± SD. Two-tailed t tests were used to generate P-values. *P < 0.05, ^ϕP < 0.1. Source data are provided as a Source Data file.