Morphological characteristics of the triple-optimized PRP + CPC construct. (A) Gross observation of PRP with 4000 × 109 pl/L (B, C) Flow cytometry analysis of platelet membrane markers, i.e., CD41a and CD42b. (D, E) Ultrastructure of platelets presented by the TEM analysis. Scale bars in graphs (D) and (E) are 4 μm and 1 μm, respectively. The white arrow indicates α-particles in platelets. (F) Gross appearance of the PRP gel. G, H) Histological morphology of the PRP gel. Scale bars represent 200 μm and 50 μm in graphs (G) and (H), respectively. (I, J) Ultrastructure of the PRP gel. Scale bars in graphs (I) and (J) are 100 μm and 40 μm, respectively. (K) Gross observation of the triple-optimized PRP + CPC construct. (L, M) Histological morphology of the triple-optimized PRP + CPC construct. Scale bars in graphs (L) and (M) are 100 μm and 25 μm. (N, O) Ultrastructure of the triple-optimized PRP + CPC construct. Scale bars in graphs (N) and (O) are 10 μm and 2 μm.