Fig. 6.

Cartilage regeneration of the optimized PRP + CPC constructs after 4-week cultivation in vitro. (A) HE staining of CPC construct, PRP + CPC construct, biochemical PRP + CPC construct, biomechanical PRP + CPC construct, triple-optimized construct, and chondrogenic induction construct post-28-day cultivation in vitro. The chondrogenic induction group was considered a positive control. Scale bars = 1 mm. (B–D) Representative sections stained with Toluidine Blue, Safranin-O, and Alcian Blue staining in the 6 groups at 4 weeks. Scale bars = 1 mm. (E, F) Measurement of the regenerated tissue area and evaluation of the histological scores based on the visual scoring system (Bern Score). Data are presented as mean ± SEM, n = 3, *P < 0.05, **P < 0.01, ***P < 0.001 and ^#P > 0.05. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)