Figure 5.
Time-dependent inhibition of the PA endonuclease activity of FluB-ht by BXA (I).A, schematic representation of the experimental setup. B, inhibition of WT FluB-ht by BXA increases with the duration of BXA preincubation (C) Inhibition of PA I38T FluB-ht by BXA is time-independent. Error bars represent the standard deviation of at least three independent experiments. All experiments were performed under the following final conditions: 100 nM 20-nt substrate, 30 mM Tris-pH 7.5, 5% DMSO, 25 mM NaCl, BXA as specified, 55 nM FluB-ht, 1.7 μM vRNA, and 5 mM MgCl2.