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. 2021 Mar 3;296:100505. doi: 10.1016/j.jbc.2021.100505

Figure 4.

Figure 4

Preconditioning with anoxia does not increase the folding proficiency of LDLR. HeLa cells were seeded and transfected with LDLR the next day. Twenty-four hours thereafter, cells were exposed to 0% O2 for 18 h (“0% O2 Pre-treatment”), followed by 35S pulse labeling for 30 min under the indicated % O2. Following the indicated chase times, LDLR was immunoprecipitated (IP) from total cell lysates and resolved by nonreducing (NR) SDS-PAGE (A). B, the fraction of LDLR in Golgi was calculated after densitometry of autoradiography (average ± SD, n = 2).