Figure 7.

Illustration of oxidative folding fitness in hypoxia for four ER cargo. Oxidative folding fitness in 0% O₂ was assigned as the percentage of the protein that achieves a conformation similar to the one in normoxia for the longest time point experimentally assessed. Data for LDLR and Flu-HA are from Fig. 2A and Koritzinsky et al. (8). Data for CA9 and VEGF-A are from Figures 3B and 2C respectively. Proteins represented by a red box (VEGF-A and CA9) are transcriptionally induced by hypoxia. Folding fitness in hypoxia varies widely between different proteins, and hypoxia-induced proteins tend to fold better in the absence of oxygen.