Table 1.
Excreted end products from metabolism of [U-13C]-glucose and nonenriched proline by BSF parental and mutant cell lines
| Excreted end products (carbon source) | WT |
Δpepck |
Δpepck∗ |
Δpepck∗/OEFRDg.nia |
Δpepck∗/OEFRDg.ia |
Δpepck∗/RNAiFRDg-m2.ni |
Δpepck∗/RNAiFRDg-m2.i |
|---|---|---|---|---|---|---|---|
| nmol·h−1·108 cells−1b | |||||||
| nc | 3 | 3 | 3 | 3 | 3 | 3 | 3 |
| Succinate (glucosed) | 277 ± 15 | ND | ND | ND | ND | ND | ND |
| Succinate (prolined) | 132 ± 19 | 147 ± 21 | 138 ± 14 | 94 ± 7 | 78 ± 6 | 78 ± 4 | 72 ± 4 |
| Acetate (glucose) | 1431 ± 121 | 385 ± 25 | 400 ± 39 | 414 ± 32 | 409 ± 10 | 402 ± 20 | 394 ± 11 |
| Acetate (proline) | 238 ± 12 | 366 ± 26 | 395 ± 39 | 308 ± 16 | 330 ± 18 | 345 ± 19 | 347 ± 15 |
| Alanine (glucose) | 229 ± 25 | 374 ± 29 | 405 ± 41 | 367 ± 23 | 391 ± 13 | 409 ± 14 | 400 ± 7. |
| Alanine (proline) | 31 ± 4 | 142 ± 11 | 156 ± 20 | 163 ± 8 | 182 ± 15 | 140 ± 15 | 141 ± 8. |
| Total (glucose) | 1950 ± 120 | 759 ± 54 | 806 ± 78 | 781 ± 54 | 800 ± 12 | 811 ± 34 | 794 ± 16 |
| Total (proline) | 401 ± 15 | 655 ± 56 | 689 ± 71 | 565 ± 29 | 590 ± 15 | 563 ± 28 | 561 ± 20 |
ND, not detectable.
a
i: RNAi cell line induced during 5 days by addition of tetracycline;.ni: noninduced RNAi cell line.
b
The amounts of end products excreted from glucose and proline metabolism are expressed as nmoles excreted per hour and per 108 cells.
c
Number of biological replicates.
d
Carbon source metabolized into succinate.