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. 2021 Mar 18;296:100563. doi: 10.1016/j.jbc.2021.100563

Figure 1.

Figure 1

p38α loss does not rescue aging phenotypes in hematopoietic cells in 1-year-old mice.A, experimental design used to test effects of p38α loss in 1-year-old mice. B and C, analysis of PB differentiation status (B220+ B cells [B], CD4+ T cells [CD4], CD8+ T cells [CD8], Mac-1+Gr-1hi granulocytes [Gra] or Mac-1+Gr-1lo macrophages [Mac]). B, frequencies of BM HSPCs, including CLPs (common lymphoid progenitors; Lin−IL7Rα+Flt3+Sca-1/c-Kitlo), MEPs (megakaryocyte-erythroid progenitors; Lin−IL7Rα−Sca-1−c-Kit+CD16/32−CD34−), GMPs (granulocyte-monocyte progenitors; Lin−IL7Rα−Sca-1−c-Kit+CD16/32+CD34+), CMPs (common myeloid progenitors; Lin−IL7Rα−Sca-1−c-Kit+CD16/32−CD34+), MPPs (CD34+Flt3+LSK), ST-HSCs (CD34+Flt3−LSK), or LT-HSCs (CD34−Flt3−LSK) (C) in 1-year-old control p38α+/+ or mutant p38αΔ/Δ mice (means ± SD, n = 5). D and E, donor-derived chimerism (D) and lineage differentiation (CD4/8+ T cells [T], B220+ B cells [B], or Gr-1/Mac-1+ myeloid cells [myeloid]) status (E) of PB cells during primary BMT of LT-HSCs from 1-year-old p38α+/+ or p38αΔ/Δ mice (mean ± SE, n = 4–6). F, donor-derived chimerism of BM cells, including LT-HSCs (CD34−Flt3−LSK), ST-HSCs (CD34+Flt3−LSK), MPPs (CD34+Flt3+LSK), LKS− (Lin−c-Kit+Sca-1−), Lin+ (lineage marker positive), and PI− (propidium iodide negative) cells from primary recipients at 4 months post-BMT (mean ± SD, n = 5–6). G and H, donor-derived chimerism (G) and lineage differentiation status (H) of PB cells after secondary BMT of LT-HSCs from 1-year-old p38α+/+ or p38αΔ/Δ mice (mean ± SE, n = 4–6). I, donor-derived chimerism of BM cells, including LT-HSCs (CD34−Flt3−LSK), ST-HSCs (CD34+Flt3−LSK), MPPs (CD34+Flt3+LSK), LKS− (Lin−c-Kit+Sca-1−), Lin+ (lineage marker positive), and PI− (propidium iodide negative) cells, from secondary recipients at 4 months post-BMT (mean ± SD, n = 5–6). J, donor-derived chimerism of PB cells after tertiary BMT of LT-HSCs from 1-year-old p38α+/+ or p38αΔ/Δ mice (mean ± SE, n = 4–6). All data are derived from a single experiment. ∗p < 0.05, ∗∗p < 0.01. BM, bone marrow; BMT, bone marrow transplantation; HSPC, hematopoietic stem/progenitor cell; LT-HSC, long-term hematopoietic stem cell; MPP, multipotent progenitor cell; PB, peripheral blood; ST-HSC, short-term hematopoietic stem cell; TAM, tamoxifen.